BioProfile® FAST CDV
FASTEST Fully Automated Cell Density/Viability Analyzer
• Over 45 samples per hour throughput
• Small, 100μL samples
• Automated cell counts to 140e6 vc/mL
• 32-position carousel tray and 96 well plate sampling options
• 21 CFR Part 11 compliant software
• Full network integration – transmits data and images
About
BioProfile FAST CDV is a fully automated analyzer designed to meet the needs of cell culture scientists requiring high throughput, rapid and extremely accurate cell density and cell viability measurements. Utilizing trypan blue dye exclusion methodology with the industry’s most innovative and automated sampling preparation, results are available in just one minute while eliminating virtually all manual sample prep procedures. Two precision syringe pumps perform all necessary dilution steps enabling fully automated cell counts to 140e6 vc/mL. FAST CDV requires just 100μL of sample volume which can be analyzed using Nova’s proprietary sample cups designed specifically to eliminate adherence of cells to the cup walls and to enhance mixing of high cell density cultures.
Sampling options
Sampling options include a 32-position carousel tray with true load-and-go functionality and a 96-well motorized tray. With the carousel tray, samples can be continuously loaded without having to remove spent cups, providing maximum workflow efficiency. An onboard waste receptacle has a storage capacity of 300 cups. The 96 well tray accepts standard 96 well plates for processing.
32-position carousel tray with over 45 samples per hour throughput
96-well plate sampling options
Automated maintenance
No maintenance is required beyond reagent pack replacements. All fluid path maintenance is performed automatically utilizing cleaning solutions contained within the reagent pack to ensure maximum uptime.
High-capacity, cartridge-based reagent management system
FAST CDV reagent cartridges incorporate RFID technology for auto-recognition of pack installation as well as logging the installation date, time, lot number, expiration date, and quantity of tests available. Reagent packs have a use life of more than 500 samples and up to 60 days once installed.
Full network capabilities
FAST CDV has a multitude of options for data transmission and IT data integrity. Both raw data and images can be sent to user-defined network locations on your existing network. As an OPC-compliant device, a custom OPC server coupled with FAST CDV can add an additional level of encryption while also enabling bi-directional communication. Windows Active Directory is used to simplify user account management and user profiles.
Maintenance-free microsensor cards
Nova has also linked the FLEX2 analyzer with ambr 15 and 250 microbioreactors to give a fully automated solution. The External Sampling Module (ESM) of FLEX2 automatically transports cell culture samples from ambr® 250 and ambr® 15 microbioreactor systems to FLEX2 for analysis.
Guaranteed Measurement Accuracy
The RMS from FLEX2 includes smart reagent cartridges that are simple to use and refill.
• RMS records the date and time of cartridge installation, as well as the lot number and expiration date.
• A self-contained trash receptacle within the cartridge minimises direct waste handling and direct interaction with hazardous trypan blue and biological contaminants, maximising analyzer readiness. Other systems can expose operators to considerable waste management risks.
Specifications
Physical Dimensions:
Width: 17.5”, Depth: 21.5”, Height: 16.5”
Parameter Linear Range:
Measurement range: 50,000 cells/mL to 140,000,000 cells/mL.
This range is accomplished by user-selectable onboard dilutions performed by the analyzer.
No external/manual dilutions are required. User selectable dilution ratios:
Ratio 1:1 0.05 to 35.00 million cells/mL
Ratio 1:2 0.20 to 70.00 million cells/mL
Ratio:1:4 0.40 to 140.00 million cells/mL
Viability Range:
0 to 100%
Live Cell Diameter Range:
4 to 70 μm
| Accuracy Specifications Summary | ||||
| Parameter | Reference Analyzer | R2 | Lower Slope Limit | Upper Slope Limit L |
| Total Cell Density | BioProfile FLEX2 | 0.97 | 0.95 | 1.05 |
| Viable Cell Density | BioProfile FLEX2 | 0.97 | 0.95 | 1.05 |
| Viability (%) | BioProfile FLEX2 | 0.97 | 0.95 | 1.05 |
| Live Cell Diameter | BioProfile FLEX2 | 0.97 | 0.95 | 1.05 |
| Units of Measure and Resolution | ||
| Analyte | Resolution | Alternate Units |
| Viable Cell Density | 0.01 e6 cells/mL | 0.01 e5 cells/mL |
| Total Cell Density | 0.01 e6 cells/mL | 0.01 e5 cells/mL |
| Viability | 0.1 % | |
| Cell Diameter | 0.01 μm | |
| % Aggregate | 0.1 % |